By John M. King (auth.), Walter L. Smith, Matoira H. Chanley (eds.)
This quantity is predicated on prec'entations on the convention on tradition of Marine Invertebrate Animals which used to be held in eco-friendly port, long island in October, 1972. The convention was once subsidized via the center Atlantic traditional Sciences Council, Inc., a non revenue academic company, including the Marine technological know-how facilities of Adelphi collage, the country college of recent York at Stony Brook, ny collage, Suffolk County group collage, and the guard Island Oyster corporation. the aim of the convention used to be to supply a wanted ex swap of information between scientists of assorted specialties whose info will be worthy to others faced with comparable difficulties, despite diversified marine animals. half I considers supportive ideas -- basic isolation and tradition tools, difficulties of affliction and feeding. particular options hired within the tradition of quite a lot of invertebrate organisms is roofed partially II. we wish to thank the members for his or her cooperation in getting ready the manuscripts in keeping with their convention shows. Walter L. Smith Matoira H. Chanley v Contents half I Recirculating process tradition tools for Marine Organisms .............•.
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Extra resources for Culture of Marine Invertebrate Animals: Proceedings — 1st Conference on Culture of Marine Invertebrate Animals Greenport
Most algae grow without added buffer. The bicarbonate content of the medium can be increased. (Note that seawater has 2 roM bicarbonate). 6 mg B or 24 mq H1B03 per liter. Boron is not present in the trace metal mix (Taoles 6 and 7) because this element is abundant in seawater. However, if seawater is diluted tenfold or more, the possibility of a boron deficiency exists, at least for some diatoms. In any event, boron is presumably re'luired for the freshwater media (though it is 'luite possible that enough is provided by borosilicate glass).
TABLE S Vitamin Primary Stock Solutions and Vitamin Working Stock Solutions Primary stock solutions: Biotin is obtained in crystalline form; allow for about 4% water of crystallization. 6 ml for each mg of biotin. Make the solution slightly acid if it is to be autoclaved. Keep the solution sterile and frozen. S3 PHYTOPLANKTON FOR FEEDING MARINE INVERTEBRATES Vitamin B12 is similarly obtained as crystals and ll% should be allowed for water of crystallization. Weigh (or buy in weighed amounts) and make a primary stock solution having 1 mg/ml.
Moore, Editor. Part H. Brachiopoda. , Lawrence, Kansas; University Kansas Press & Geol. Soc. America. CULTURE OF PHYTOPLANKTON FOR FEEDING MARINE INVERTEBRATES Robert R,L, Guillard Biology Department, Woods Hole Oceanographic Institution Woods Hole, Massachusetts These pages describe relatively simple and reliable methods for the culture of marine phytoplankton species useful for feeding marine invertebrates. The methods suffice for the most fastidious algae now routinely cultivable, and simplifications indicated for less demanding species are easily made; for example, omission of s11icate for plants other than diatoms, Certain modifications of techniques, ancillary methods, and precautions will be treated briefly because questions often arise concerning them, but documentation will be minimal and hopefully restr~cted to publications readily available, The methods apply to the production of sterile cultures of considerable density in volumes up to 18 liters, in commercially available 5 gallon borosilicate glass carboys, Apparatus for growing unialgal (not bacteria-free) cultures in volumes up to 160 liters has been described (Ukeles, 1971; Siegelman and Guillard, 1971), Such cultures, started from sterile carboy cultures, can be used in turn to inOCUlate larger outdoor tank or pond cultures, This is another problem entirely, All non-sterile cultures suffer the disadvantage that they may become contaminated with bacteria that injure or destroy the anjmals being fed, Larger non-sterile cultures appear to be more susceptible to invasion by competing algae and to predation by animals, It is true that carefully managed tank algal cultures as used by many hatcheries are quite reliable, but the only algal cultures suitable for critical studies are both bacteria-free and harvested while the algae are sill actively growing; that is, before maximum cell density is attained, The methods to be described take an algal culture to the point of usefulness for experiment, for maintenance of relatively 29 30 ROBERT R.