Chromatographic Fingerprint Analysis of Herbal Medicines:

This guide, to be released in volumes, offers a condensed evaluate of the analytical research of eighty chinese language natural medications that are most often in use.

Thin layer chromatographic-, excessive strain liquid chromatographic- and fuel chromatographic-fingerprint analytical ideas permit the detection of all major low-molecular components of a plant drug or even unmarried parts may be visualized. Analytical effects thereof are proven in different colour figures.

The caliber facts of the research meets the traditional of the ecu Drug Regulatory Authority. additionally, this quantity provides an in depth description of the analytical tools used for a number of medications. Bioactive components, pharmacological and organic actions of numerous unmarried natural medications in addition to their healing functions are mentioned.

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Additional info for Chromatographic Fingerprint Analysis of Herbal Medicines: Thin-Layer and High Performance Liquid Chromatography of Chinese Drugs, Volume 1 (2nd Edition)

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A. ). 1,0 ml/min. ) Compounds 1 2 3 4 5 6 7 8 10,7 12,6 13,1 15,1 20,9 34,4 35,5 43,3 saikosaponin c saponin (non identi¿ed) saponin (non identi¿ed) saikosaponin a saikosaponin d polyacetylene polyacetylene sterol 7 Radix Bupleuri – Chaihu Fig. 3: UV-spectra of the major compounds Fig. 4: HPLC ¿ngerprint chromatogram of a drug sample of Chinese origin, Hebei Province (North) (sample 3). ) at Rt 12,6, 13,1 (2,3) are present in detectable quantities. The polyacetylene compounds at Rt 34,4 (6) and Rt 35,5 (7) are present at low concentrations.

In vivo effects(3, 4, 15) – antihepatotoxic (rats, humans) – antipyretic (rabbits, mice, rats) – analgesic (saponins and saikogenin A) (mice) – anti-inÀammatory (rat-paw edema model) – antigranulomatotic in rats – sedative (saponins and saikogenin A) (mice) – cholagogue and choleretic (whole-plant extract) (dog) – anticholesterolemic (saikosaponin a, d and genin A, D (rats and rabbits) – anti-ulcerogenic (rats) – antihypertensive 2 Radix Bupleuri – Chaihu Fig. a. for 4 hrs. The clear extract is concentrated to approx.

Expectorant effects: alkaloid fraction and Fritillaria-saponins in mice(3). – hypotensive effect: verticine and verticinone in high doses; at low doses the contrary effect becomes evident(5). – sedative effect: verticine and verticinone antagonize the stimulatory effect of caffeine and potentiate the sedative effect of chlorpromazine in mice(5). In humans, preparations of Fritillariae bulbus exhibit antitussive and expectorant effects(3). Fig. 1: Formulae of the main compounds TLC ¿ngerprint analysis: 1) Extraction: 10 g coarsely-ground drug are treated for 1 hr with 50 ml 0,1 N sulphuric acid in an ultrasonic bath under occasional stirring.

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