Cell Culture Techniques in Heart and Vessel Research by A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)

By A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)

In the cardiovascular sciences, an expanding call for for using smooth equipment of phone biology has built. using particular telephone tradition versions of a number of the tissues concerned is key for many of those novel methods. This e-book meets the call for for acomprehensive and straightforward available resource for cellphone cul- ture tools in cardiovascular examine because it used to be no longer formerly to be had. the fundamental tools for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), soft muscle cells and pericytes are defined intimately by means of a global number of specialists. designated chapters speak about using progress elements and attachment substrates, innovations for co-cultures, cultures on permeable clear out membranes and microcarrier cultures. The methodological descriptions are sufficiently specified for an instantaneous program within the laboratory. All chapters additionally comprise a severe review of different approaches.

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The restoration of beating by serum lipids and fatty acids. Biochim Biophys Acta 115:15-22 31. Harary I, Hoover F, Farley B (1974) The isolation and cultivation of rat heart cells. Enzymol 32:740-745 32. Harary I, Renaud J-F, Wallace G (1976) Ca ions regulate cyclic AMP and beating in cultured heart cells. Nature 261:60-61 33. Holtzer H (1979) Myogenesis. In: Schjeide QA, de Vellis I (eds) Cell differentiation. Van Nostrand Reinhold, New York, pp 476-503 34. Houba V (1967) The use of pronase for dispersing cells.

Pfeiffer DR, Taylor RW, Lardy HA (1978) Ionophore A23187: cation binding and transport properties. Ann NY Acad Sci 307:402-423 60. Pine L, Taylor GC, Miller DM, Bradley G, Wetmore HR (1969) Comparison of good and bad lots of trypsin used in the production of primary monkey kidney cells: a definition of the problem and comparison of certain enzymatic characteristics. Cytobios 2: 197 -207 61. Pinson A, Padieu P (1974) Erucic acid oxidation by beating heart cells in culture. FEBS Lett 39:88-90 62.

Stirring for 15 - 20 min. ICentrlfugallon I . . '--_'-'----' . . Stirring for 15 - 20 min. 1on ~ of combined ' - ~. m I .... -__ Idem -'I . . Idem I ......... 1 _Idem _----'I . . m--,I . . I I~forl celcOWII 1.... -----. ling Fig. 3. Schematic representation of culture preparation methods. [From 64] Supernatant decanted 3:: c :; n '< n i" ~ ":;. t 0- " II Neonatal Rat Heart Muscle Cells 29 Cells in each tube are suspended by repeated aspirations in a sterile pipette and all the fractions combined in a sterile 250 ml flask, passing them through a sterile mesh in order to exclude explants.

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